||I U P A C
Organizations & People
Pure Appl. Chem.,
Vol. 71, No. 5, pp. 899-918, 1999
COMMISSION ON MICROCHEMICAL TECHNIQUES AND TRACE ANALYSIS
Species-selective Analysis for Metal
Complexes using Hyphenated Techniques
Prepared for publication by J. Szpunar and R.
Abstract: Analytical chemistry
of metal complexes with biomacromolecules based on the coupling of a
high resolution separation technique with an element or species selective
detection technique is critically discussed. The role of size-exclusion
chromatography (SEC) with on-line atomic spectrometric detection is
evaluated for the characterization of the metal distribution among the
fractions of different molecular weight. Attention is given to the conditions
for the separation of metallated biomacromolecular isoforms and sub-isoforms
by anion-exchange and reversed-phase HPLC. Techniques for interfacing
chromatography with atomic absorption spectrometry (AAS), inductively
coupled plasma atomic emission spectrometry (ICP AES) and ICP mass spectrometry
(ICP MS) are assessed. The potential of electrospray (tandem) mass spectrometry
for the on-line determination of the molecular mass of the eluting protein
is highlighted. Perspectives for capillary zone electrophoresis (CZE),
microbore and capillary HPLC with ICP MS and electrospray MS detection
for probing metalloproteins are discussed. Applications of hyphenated
techniques to the analysis of real-world samples are reviewed.
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